Inicio > Matemáticas y ciencia > Biologia, ciencias de la vida > Ciencias de la vida: cuestiones generales > Genética (no médica) > Multipleksowa analiza topnienia o wysokiej rozdzielczości jako narzędzie diagnostyczne
Multipleksowa analiza topnienia o wysokiej rozdzielczości jako narzędzie diagnostyczne

Multipleksowa analiza topnienia o wysokiej rozdzielczości jako narzędzie diagnostyczne

Kais Kassim Ghaima / Shahad Tariq Hamad

96,42 €
IVA incluido
Disponible
Editorial:
KS OmniScriptum Publishing
Año de edición:
2024
Materia
Genética (no médica)
ISBN:
9786208384807
96,42 €
IVA incluido
Disponible
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Badanie to miało na celu opracowanie multipleksowego RT-PCR z analizą topnienia w wysokiej rozdzielczości (HRM) w celu szybkiego i dokładnego wykrywania pięciu genów utrzymujących karbapenemazy 16S rRNA. Sześć par starterów i warunki RT-PCR zostały zaprojektowane zgodnie z oprogramowaniem Beacon Designer. Identyfikację genów przeprowadzono za pomocą krzywych topnienia i temperatur za pomocą Multiplex RT-PCR przy użyciu dedykowanego odczynnika HRM. Jest to pierwszy test wykrywający główne geny karbapenemazy w teście dwururowym, umożliwiający jednoczesne wykrywanie 5 najważniejszych markerów oporności na cefalosporyny 3. generacji i karbapenemy.

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Otros libros del autor

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  • L’analyse de fusion multiplex à haute résolution comme outil de diagnostic
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    O objetivo deste estudo foi desenvolver um RT-PCR multiplex com análise de fusão de alta resolução (HRM) para a deteção rápida e exacta de cinco carbapenemases com o gene 16S rRNA. Foram concebidos seis pares de primers e condições de RT-PCR de acordo com o software Beacon designer. A identificação dos genes foi efectuada através de curvas de fusão e temperaturas com Multiplex ...
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